Journal: International Journal of Molecular Sciences

Article Title: Simultaneously Targeting Two Coupled Signalling Molecules in the Mesenchymal Stem Cell Support Efficiently Sensitises the Multiple Myeloma Cell Line H929 to Bortezomib

doi: 10.3390/ijms24098157

Figure Lengend Snippet: BM-MSC are able to recover H929 cell viability after strong BTZ treatment. ( A ) H929 cells were treated with different concentrations of BTZ for different periods of time, as indicated. Cell viability was assessed by the MTT assay. ( B ) H929 cells were pre-treated with different concentrations of BTZ for 72 h, washed and then co-cultured or not with BM-MSC for 24 h. Pre-treated H929 cells cultured in IMDM with 10% FBS for 24 h were used as controls. Data are expressed as the mean ± SEM of triplicate cultures. Statistical analysis was performed using a one-way ANOVA test followed by a Kruskal–Wallis test ( A ) and a two-way ANOVA followed by Dunnett’s post hoc test ( B ). No significant statistical differences (ns) and p -values ** < 0.01, *** < 0.001, **** < 0.0001.

Article Snippet: 1 or 5 × 10 4 BM-MSC/well were seeded in complete IMDM medium in 96 or 24 well plates, respectively (SPL Life Sciences Co., Gyeonggi-do, Korea) depending on the experiment and incubated for 24 h in standard conditions to let them adhere.

Techniques: MTT Assay, Cell Culture